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The Kunitz area could exist singly, or as several domains in a single polypeptide [three]. numerous Kunitz domains work as protease inhibitors by way of their scissile bond at positions 15 (P1) and 16 (P1′). The P1 residue is commonly a simple amino acid (K or R) although the P1′ position is an A or G, which jointly connect with the Energetic w